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Bacteriological Agar is used as a solidification agent in the preparation of microbiological culture media.

Bacteriological Agar is a water soluble colloidal extract obtained from certain species of marine red algae including Gelidium, Pterocladia, and Gracilaria. Impurities, debris, minerals, and pigment are reduced to specific levels during manufacture. The majority of microorganisms cannot digest agar thus making it an excellent solidifying agent. The addition of 13-15 grams/Liter is recommended for the preparation of most dehydrated culture media formulations. If the culture medium has a pH of 6 or less, 20 grams/Liter should be used. Semi-solid medium is obtained with agar concentrations of 3-8 grams/Liter. Bacteriological Agar is optimized for best magnesium and calcium content. Detrimental ions such as iron and copper are reduced.