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Mueller Hinton Agar is recommended for antimicrobial disc diffusion susceptibility testing of common, rapidly growing bacteria by the Bauer-Kirby method, as standardized by the National Committee for Clinical Laboratory Standards (NCCLS). Mueller Hinton Agar with 5% Sheep Blood is recommended for antimicrobial disc diffusion susceptibility testing of Streptococcus pneumoniae with selected agents; i.e., chloramphenicol, erythromycin, ofloxacin, tetracycline, and vancomycin, in addition to oxacillin screening for susceptibility to penicillin, as standardized by the NCCLS.

Description: Acid hydrolysate (digest) of casein and beef extract supply amino acids and other nitrogenous substances, minerals, vitamins, carbon, and other nutrients to support the growth of microorganisms. Starch acts as a protective colloid against toxic substances that may be present in the medium. Hydrolysis of the starch during autoclaving provides a small amount of dextrose, which is a source of energy. Agar is the solidifying agent